A new method for construction of Ti plasmid-less strains in Agrobacterium tumefaciens.
نویسندگان
چکیده
Agrobacterium tumefaciens harboring a Ti plasmid causes plant tumorigenesis and it's DNA transfer system have been extensively used in plant biotechnology. Because Ti plasmids are high stable in agrobacterial hosts, their curing is very difficult by conventional methods. Here, we introduced a novel curing method by using Ti plasmids incompatibility.
منابع مشابه
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عنوان ژورنال:
- Nucleic acids research. Supplement
دوره 1 شماره
صفحات -
تاریخ انتشار 2001